제목 | Reciprocal Crosses Between Astaxanthin and Capsanthin Rice Unravel Effects of Metabolic Gene Efficacy in Rice Endosperm |
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내용 |
Carotenoids are not naturally accumulated in the endosperms of rice, which is a cereal crop. In our previous study, biofortifed rice seeds accumulated two red color carotenoids, ketocarotenoids including astaxanthin and ketoxanthophylls including
capsanthin, via four-step pathway engineering. Their biosynthetic pathways require zeaxanthin as a common precursor and
then bifurcated. In this study, cross-fertilization between two astaxanthin (BP and sBP) and two capsanthin (C and CB) rice
plants were reciprocally performed to elucidate the rice seed system’s metabolic preference. Filial seeds showed diferent
colors in red hues between mutually interbred lines as well as from parents. The strongest driving force for metabolism
was caused by the sBP line for astaxanthin regardless of the parent side and proved to be the most efcacious customized
synthetic gene for rice crops. It further resulted in the highest astaxanthin levels when paternal sBP mated with maternal CB
line, which has the biggest capacity of total carotenoids. Also, reciprocal crosses between CB and BP lines ascertained their
metabolically balanced gene efcacy by showing the pathway preference toward either ketoxanthophylls or ketocarotenoids
depending on the paternal parental side. Our study suggests that gene efcacy plays the most decisive role in leading the
metabolic path and revealed the unexpectedly paternal parent-of-origin efects in the endosperm. Consequently, we simultaneously biofortifed rice crops with two functional red carotenoids and fnally generated new red-colored
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첨부 |
2021 Jeong et al..pdf
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