제목 | Comparative Genomics Reveals a Remarkable Biosynthetic Potential of the Streptomyces Phylogenetic Lineage Associated with Rugose-Ornamented Spores |
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내용 |
The genus Streptomyces is one of the richest sources of secondary metabolite biosynthetic gene clusters (BGCs). Sequencing of a large number of genomes has
provided evidence that this well-known bacterial genus still harbors a large number of
cryptic BGCs, and their metabolites are yet to be discovered. When taking a gene-first
approach for new natural product discovery, BGC prioritization would be the most crucial step for the discovery of novel chemotypes. We hypothesized that strains with a
greater number of BGCs would also contain a greater number of silent unique BGCs
due to the presence of complex regulatory systems. Based on this hypothesis, we
employed a comparative genomics approach to identify a specific Streptomyces phylogenetic lineage with the highest and yet-uncharacterized biosynthetic potential. A comparison of BGC abundance and genome size across 158 phylogenetically diverse
Streptomyces type strains identified that members of the phylogenetic group characterized by the formation of rugose-ornamented spores possess the greatest number of
BGCs (average, 50 BGCs) and also the largest genomes (average, 11.5 Mb). The study of
genetic and biosynthetic diversities using comparative genomics of 11 sequenced
genomes and a genetic similarity network analysis of BGCs suggested that members of
this group carry a large number of unique BGCs, the majority of which are cryptic and
not associated with any known natural product. We believe that members of this
Streptomyces phylogenetic group possess a remarkable biosynthetic potential and thus
would be a good target for a metabolite characterization study that could lead to the
discovery of novel chemotypes.
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첨부 |
2021 Chung et al..pdf
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