제목 | Molecular Characterization of U-box E3 Ubiquitin Ligases (TaPUB2 and TaPUB3) Involved in the Positive Regulation of Drought Stress Response in Arabidopsis |
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내용 |
Plant U-box E3 ubiquitin ligase (PUB) is involved in various environmental stress conditions. However, the molecular mechanism of U-box proteins in response to abiotic stress in wheat
remains unknown. In this study, two U-box E3 ligase genes (TaPUB2 and TaPUB3), which are highly
expressed in response to adverse abiotic stresses, were isolated from common wheat, and their
cellular functions were characterized under drought stress. Transient expression assay revealed
that TaPUB2 was localized in the cytoplasm and Golgi apparatus, whereas TaPUB3 was expressed
only in the Golgi apparatus in wheat protoplasts. Additionally, TaPUB2 and TaPUB3 underwent
self-ubiquitination. Moreover, TaPUB2/TaPUB3 heterodimer was identified in yeast and the cytoplasm of wheat protoplasts using a pull-down assay and bimolecular fluorescence complementation
analysis. Heterogeneous overexpression of TaPUB2 and TaPUB3 conferred tolerance to drought
stress. Taken together, these results implied that the heterodimeric form of U-box E3 ubiquitin
ligases (TaPUB2/TaPUB3) responded to abiotic stress and roles as a positive regulator of drought
stress tolerance.
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첨부 |
2021 Kim et al..pdf
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