제목 | Development of subgenome-specific PCR markers in the short arm of wheat and rye chromosome 1 and their utilization in wheat-rye translocation breeding. |
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내용 |
T1RS.1BL wheat-rye translocation has
been widely used in wheat breeding owing to
agronomically beneficial genes located on the short
arm of rye chromosome 1 (1RS). Although numerous
methods have been developed to identify wheat lines
possessing 1RS, there are some limits to their practical
application in large breeding populations. In this
study, we developed 10 homoeologous markers
specific to each short arm of chromosome 1 of
common wheat and rye based on genome databases,
and their specificity was validated using wheat aneuploid and rye chromosome addition lines. Furthermore, recombinant inbred lines (RILs) were
developed by crossing T1RS.1BL and non-translocated wheat and screening with the developed markers. Seed storage protein analysis revealed that the
RILs possessed high gluten quality, and recombination had occurred in gluten and gliadin gene coding
loci, except for in the 1RS region. The root and shoot
biomasses of the RILs under drought and recovery
were measured, indicating superior drought tolerance
of the T1RS.1BL lines. The markers and RILs
developed in this study could be useful in breeding
wheat-rye translocations
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첨부 |
2021 Jung and Seo.pdf
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